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Folding of E. coli (□) and T. thermophilus (▪) S-domains monitored by CD (top panel) and absorbance (bottom panel) at 287 nm (0.3 μM RNA, 37°C, 20 mM Tris at pH 8.1). The inset shows a plot for titrations performed at various [urea] to determine the m-value. Changes in absorbance and CD track the U-to-I transition and the I-to-N transition, respectively.
