Skip to main content
. 2006 Apr;12(4):567–579. doi: 10.1261/rna.2251306

TABLE 1.

Statistics of the 10 lowest energy structures

Number of restraints used in structure calculations
NOE distance 218
Experimentally derived 113
    (residues 7–17, 28–35) 99
    (intranucleotide) 36
    (intermolecular RNA-GTP) 14
    Total involving exchangeable protons 47
Model-derived A-form stems
    (residues 1–6, 18–20, 25–27, 36–41) 77
Model-derived UUCG tetraloop
    (residues 21–24) 28
Dihedral angle 149
Dipolar couplings 25
Stem hydrogen bonds 42
Total no. of restraints 434
Average rmsd from experimental restraints
    Distance (no violations >0.2 Å) 0.02 Å
    Dihedral (no violations >5°) 0.14°
    RDC (no violations >5 Hz) 1.6 Hz
Average rmsd from ideal geometries
    Bonds 0.006 Å
    Angles 1.21°
    Impropers 0.74°
Heavy atom rmsd from mean structure
    All residues (1–41, GTP) 3.38 ± 0.77 Å
    Recognition bulge and ligand (residues 8–16, 29–34, GTP) 1.97 ± 0.34 Å
    Recognition bulge and ligand without 1.47 ± 0.38 Å
    unconserved U’s (see text) (residues 8, 9, 11, 12, 14–16, 29, 31–34, GTP) Recognition bulge alone without 1.47 ± 0.36 Å
    unconserved U’s (see text) (residues 8, 9, 11, 12, 14–16, 29, 31–34) Ligand alone (GTP) 1.35 ± 0.37 Å
HHS Vulnerability Disclosure