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Cells lacking Lhp1p require TRM1 for growth at 37°C. (A) Sites of TRM1, TRM2, TRM3, PUS3, and PUS4 modification are shown on a diagram of a generic tRNA (based on Hopper and Phizicky 2003). (B) Serial dilutions of wild-type cells (TRM1 LHP1), cells lacking Lhp1p (TRM1 lhp1Δ), cells lacking Trm1p (trm1Δ LHP1), and cells lacking both Lhp1p and Trm1p (lhp1Δ trm1Δ) were spotted on YPD agar and grown at the indicated temperatures.
