Figure 4. Production of interleukin 12 (IL-12) was measured in CT26 cells infected with NV1042 at multiplicities of infection (MOIs) of 0.1, 1, and 5. Supernatants from cultures of infected CT26 cells were harvested at varying time points after infection. IL-12 concentrations were assayed by enzyme-linked immunosorbent assay to determine cumulative IL-12 production (mean ± standard deviation). The highest viral titer (MOI = 5) produced the most initial IL-12, but significant toxicity at this dosage caused an early plateau of cytokine production. At the lower MOI = 1, a slight delay in the IL-12 peak was noted before viral replication and IL-12 production occurred. Eventual toxicity at this dose resulted in a plateau as well. The least toxic MOI (0.01) resulted in significant IL-12 production, although at a later time point after viral replication occurred.