Fig. 1.

Caja-DRB alleles detected in a panel of 15 common marmosets. Shading is used to indicate whether the respective allele is detected only on the genomic (exon 2) level (black shading), or on both the genomic and cDNA levels (gray shading). Animals m02040 and m02041 were tested only on cDNA. Because it was impossible to develop -DRB lineage-specific primers that did not crossreact with other DRB cDNAs, real-time PCR results were not reliable. Therefore, transcription levels were determined by counting the number of clones. For every individual, at least 60 clones were analyzed. All transcripts of DRB*W16 and of the DRB1*0307/DRB1*0312 alleles were detected in at least 50% of the clones, and DRB*W12 alleles were detected in ≈25% of the clones. The transcripts of DRB1*03L alleles, however, were found at a much lower extent, namely in <5% of the clones tested.