Figure 5. RT-PCR analysis of adrenocortical enzymes and StAR at 8 wpc.

(A) RT followed by 22 and 28 cycles of PCR for transcripts encoding steroidogenic enzymes. (B) Specific identification of HSD3B2 and HSD3B1 isoforms in the presence (+) and absence (–) of RT. No HSD3B1 transcript was detected in the adrenal sample after 42 cycles of PCR. Positive controls were fetal testis (HSD3B2) and skin (HSD3B1); negative controls were H₂O and genomic DNA (G).