Figure 6. The release of mast cell renin in isolated mouse hearts subjected to ischemia/reperfusion is associated with NE release and reperfusion arrhythmias.

Nineteen mouse hearts were subjected to 20-minute ischemia (i.e., perfusion with glucose- and pyruvic acid–free KH buffer bubbled with 95% N₂ + 5% CO₂) followed by a 30-minute reperfusion with oxygenated KH buffer, either in the absence (n = 7) or presence of cromolyn (300 μM; n = 6) or BILA2157 (100 nM; n = 6). Bars indicate means α SEM. (A) Duration of reperfusion arrhythmias (VT/VF). Note that reperfusion arrhythmias were prevented in hearts pretreated with BILA2157. (B and C) Overflow of NE and Ang I–forming activity collected over 30 minutes either before ischemia or at the start of reperfusion. *Significantly different from own control; P < 0.05 by 1-way ANOVA with Dunnett’s produre.