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. Author manuscript; available in PMC: 2006 Mar 31.
Published in final edited form as: Nature. 2005 Jul 28;436(7050):563–567. doi: 10.1038/nature03849

Figure 2.

Figure 2

The Jak/Stat pathway is activated by the male somatic gonad. Embryos are Stage 15 and wild type unless noted. a–d, anti-Vasa (red), anti-STAT92E (green). a,b, Males stage 11 (a) and stage 13 (b). Gonad (outline) and mis-localized germ cells (arrows) are indicated. c, Mis-localized germ cells in male wunenCE mutant (inset: germ cells in the gonad of the same embryo). d. XX germ cells in a male soma (dsxD/dsx1). e,f, upd riboprobe (red) and anti-Vasa (green). upd is expressed in (e) male and not (f) female gonads. g,h, anti-Vasa (red), anti-STAT92E (green). Gonads in (g) XY Df(os1a) embryo and (h) female embryo with germ cells expressing Upd (UAS-upd; nanos-Gal4). Scale bars (12.5μm) in a and d apply to a-c or d–h, respectively.