Table 1.
Mutagenesis primers used to examine the role of the PMV replicase motif in virus accumulation.
| Mutant | Primer | Sequence (5'-3')a |
| F313A | REP/F1-A | CCCCAGCGGCTTCGTTCTTTGC |
| F1313F2 | F1-313-F2 | GGAACCCCAGCAAACTCGTTCTTTGC |
| P317A | P317A-989R | CTGTGGGTTTTGCAACCCCAGCG |
| N323A | N323A-1007R | CAGCCAACTGGGCAGCCTCTGTG |
| L325A | L325A-1014R | CTCCAGACAGCCGCCTGGTTAGC |
| Y330A | REP/Y-A | CACACCCTGTAGAGGGCTCTCCAG |
| C335A | 1044R-C/A | CCTTTCTTATCAGCCACCCTGTAGAG |
| F357A | REP/F-A | GGAATACAGCTGGCAAGGC |
| D363A | REP/D-A | TGATCCTGGGCGTATGCGC |
| P399A | MUTPMV-1236R | GCCCCAACTAATGCATTGGTCACTAG |
| W405A | REP/W-A | CCAAGCAGTCGCATTGGCCCC |
a. The altered nucleotides on the PMV cDNA are underlined.