Table 1.
Vector ID | Vector Name | Potential Splicing Events | Description |
V1 | pDNR Dual | Splice for 3' tags | Designed for Fusions at both N- and C- termini (Clontech) |
V7 | pDNR-MCS | Splice for 3' tags | Alternate MCS that adds AscI and PacI sites to the MCS of V1 |
V37 | pDNR MCS SA | Splice for 5' and 3' tags | A splice acceptor introduced into V7 before the AscI site |
V308 | pDNR MCS lacZ alpha | Splice for 3' tags | LacZ alpha inserted between AscI and PacI sites of V7 |
V309 | pDNR MCS SA lacZ alpha | Splice for 5' and 3' tags | LacZ alpha inserted between AscI and PacI sites of V37 |
V624 | pDNR MCS BE | Splice for 3' tags | Alternate MCS with BamHI, SalI, SmaI, HindIII, EcoRI sites |
V677 | pDNR MCS BE lacZ alpha | Splice for 3' tags | LacZ alpha inserted between SalI and HindII sites of V624 |
V678 | pDNR MCS SA ES | Splice for 5' and 3' tags | Alternate MCS Splice Acceptor with AscI, EcoRI, NotI, BamHI, SalI, PacI sites. |
V795 | pDNR MCS ES | Splice for 3' tags | Alternate MCS with AscI, EcoRI, NotI, BamHI, SalI, PacI sites |
V954 | pDNR MCS SA no SA CmR | Splice for 5' and 3' tags | Silent mutations introduced in the CmR ORF of V309 to remove potential splice acceptor |
*Abbreviations used: SA – splice acceptor, MCS – multiple cloning site, CmR – chloramphenicol resistance, BE – BamHI/EcoRI, ES – EcoRI/SalI