Table 2.
acceptor vectors constructs
| Vector ID | Vector Name | Potential Splicing Events | Tag | Tag Position | Promoter | Function | Expression Verification |
| V3 | pLP-ECFP C1 (Clontech)* | none | ECFP | N-terminus | CMV | localization | Verified by Clontech |
| V4 | pLP-EGFP C1 (Clontech) | none | EGFP | N-terminus | CMV | localization | Verified by Clontech |
| V5 | pLP-EYFP C1 (Clontech) | none | EYFP | N-terminus | CMV | localization | Verified by Clontech |
| V6 | pLPS-3'EGFP (Clontech) | Splice for 3' Tags | EGFP | C-terminus | CMV | localization | Verified by Clontech |
| V956 | pLPS-3'ECFP | Splice for 3' Tags | ECFP | C-terminus | CMV | localization | Figure 3 |
| V957 | pLPS-3'ECFP-mito | Splice for 3' Tags | ECFP-mito | C-terminus | CMV | targets to mitochondria | Figure 3 |
| V1662 | pLP-RFP | none | RFP | N-terminus | CMV | localization | Additional File 5A |
| V1663 | pLPS-3'RFP | Splice for 3' Tags | RFP | C-terminus | CMV | localization | Verified, Data not Shown |
| V1664 | pLPS-3'RFP-mito | Splice for 3' Tags | RFP-mito | C-terminus | CMV | targets to mitochondria | Verified, Data not Shown |
| V25 | pLP Flag | none | Flag | N-terminus | CMV | IP/IB/IF | Additional File 5C |
| V26 | pLPS-3'Flag | Splice for 3' Tags | Flag | C-terminus | CMV | IP/IB/IF | Additional File 5B |
| V33 | pLP Flag SD | Splice for 5' Tags | Flag | N-terminus | CMV | IP/IB/IF | Additional File 5C |
| V179 | pLP Triple-Flag | none | Triple-Flag | N-terminus | CMV | IP/IB/IF | Figure 4&5 |
| V180 | pLP Triple-Flag SD | Splice for 5' Tags | Triple-Flag | N-terminus | CMV | IP/IB/IF | Figure 4&5 |
| V181 | pLPS 3' Triple-Flag | Splice for 3' Tags | Triple-Flag | C-terminus | CMV | IP/IB/IF | Figure 4&5 |
| V207 | pRETRO-Triple-Flag SD | Splice for 5' Tags | Triple-Flag | N-terminus | LTR | IP/IB/IF/retrovirus% | Additional File 5D |
| V143 | pLP-HA SD | Splice for 5' Tags | HA | N-terminus | CMV | IP/IB/IF | Additional File 5E |
| V27 | pLPS-3'HA | Splice for 3' Tags | HA | C-terminus | CMV | IP/IB/IF | Not expression verified |
| V516 | pLP-dMyc | none | Double-Myc | N-terminus | CMV | IP/IB/IF | Figure 4 |
| V517 | pLP-dMyc SD | Splice for 5' Tags | Double-Myc | N-terminus | CMV | IP/IB/IF | Figure 4 |
| V518 | pLPS-3'dMyc | Splice for 3' Tags | Double-Myc | C-terminus | CMV | IP/IB/IF | Figure 4 |
| V621 | pGEX-4T-LP | none | GST | N-terminus | Tac | Bacteria expression | Verified, Data not Shown |
| V622 | pGEX-2TK-LP | none | GST | N-terminus | Tac | Bacteria expression | Additional File 5F |
| V623 | pAcGHLT-LP | none | GST His | N-terminus | Ph | Baculovirus expression | Not expression verified |
| V1579 | pLP ProHis HTb | none | His | N-terminus | Trc | Bacteria expression | Additional File 5G |
*Abbreviations used: LP – loxP, mito – mitochondria localization peptide, SD – splice donor, IP – immunoprecipitation, IB – immunoblot analysis, IF – immunofluorescence, Ph – polyhedrin
%V207 has been used extensively to produce protein from transient or stably transfected cells. It has not been tested for retrovirus production or resulting protein expression from a retrovirus.