Table 1.
Efficacy of select estrogens within CEE to reduce LDH release following exposure to β-amyloid25–35 in basal forebrain neurons. Neuronal cultures were pretreated with indicated estrogens for 4 days followed by a 24 hr exposure to 8 μg/ml β-amyloid25–35. The culture medium was replaced and cultures were allowed to incubate for an additional 24 hr followed by determination of LDH release into the medium. Data are represented as mean ± SEM (n = 7 – 24 from 3 separate experiments). ** P < 0.01 and *** P < 0.001 compared to β-amyloid25–35 alone-treated cultures.
| Treatment (pg/ml) | LDH Release (% of β-amyloid25–35 alone group) |
| Control | 36.11 ± 1.60*** |
| β-amyloid25–35 alone | 100.00 ± 2.26 |
| 17α-estradiol (10) | 86.62 ± 2.99** |
| 17β-estradiol (10) | 84.20 ± 1.47*** |
| Equilin (1000) | 89.01 ± 1.74** |
| Equilenin (300) | 81.49 ± 3.06*** |
| 17α-dihydroequilin (1000) | 89.96 ± 3.88** |
| 17β-dihydroequilin (40) | 101.53 ± 2.00 |
| Estrone (5000) | 99.33 ± 4.43 |
| Δ8,9-dehydroestrone (300) | 85.82 ± 2.07*** |