Table 2.
Efficacy of select estrogens within CEE to sustain the intracellular ATP level following exposure to β-amyloid25–35 in basal forebrain neurons. Neuronal cultures were pretreated with indicated estrogens for 4 days followed by a 24 hr exposure to 8 μg/ml β-amyloid25–35. The culture medium was replaced and cultures were allowed to incubate for an additional 24 hr followed by determination of the intracellular ATP level. Data are represented as mean ± SEM (n = 7 – 24 from 3 separate experiments). ** P < 0.01 and *** P < 0.001 compared to β-amyloid25–35 alone-treated cultures.
| Treatment (pg/ml) | ATP Level (% of β-amyloid25–35 alone group) |
| Control | 203.37 ± 11.67*** |
| β-amyloid25–35 alone | 100.00 ± 2.07 |
| 17α-estradiol (10) | 103.83 ± 3.76 |
| 17β-estradiol (10) | 131.32 ± 4.56** |
| Equilin (1000) | 114.58 ± 3.56 |
| Equilenin (300) | 96.44 ± 4.07 |
| 17α-dihydroequilin (1000) | 110.00 ± 2.75 |
| 17β-dihydroequilin (40) | 110.75 ± 2.46 |
| Estrone (5000) | 128.84 ± 4.14** |
| Δ8,9-dehydroestrone (300) | 125.05 ± 1.42** |