Table 2.

Specific activity of H-TevBmo variants

Protein	Specific activitya (U/µg)	Relative specific activityb
I-TevIc	1.4 × 104	1
I-BmoIc	1.8 × 101	∼10−3
H-TevBmoV1	1.8 × 100	∼10−4
H-TevBmoV2	4.0 × 10−1	∼10−5
H-TevBmoV3	2.8 × 10−3	∼10−7
H-TevBmoV4	2.1 × 10−3	∼10−7
H-TevBmoV5	1.5 × 101	∼10−3

^aCleavage assays were performed as described in Materials and Methods. One unit of activity is defined as the amount of enzyme required to cleave 250 ng of ScaI-linearized plasmid to 50% completion in 1 min at 37°C.

^bActivity relative to I-TevI, which was assigned a value of 1.

^cSpecific activities of I-TevI and I-BmoI were taken from Ref. (26).