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. 2006 Feb 23;25(6):1364–1374. doi: 10.1038/sj.emboj.7601004

Figure 2.

Figure 2

Expression of C3aR and C5aR on transit-amplifying precursors and migrating neuroblasts in vivo. Immunostaining of paraffin-embedded brain sections from adult mice with antibodies against C3aR (A) and C5aR (B). Both receptors are expressed by cells in the SVZ and rostral migratory stream of WT mice. The specificity of the anti-C3aR and anti-C5aR antibodies, respectively, was confirmed by the absence of immunostaining on brain sections from C3aR−/− and C5aR−/−mice. In the negative control immunostainings of WT brain sections, the primary antibodies were omitted. Olig2-positive transit-amplifying precursor cells in the SVZ are colabeled by anibodies against C3aR (C) and C5aR (D). Dcxpos migrating neuroblasts in the rostral migratory stream express C3aR (E) and C5aR (F). Arrows indicate double-labeled cells in the insert. Scale bars equal 50 μm (A, B) 10 μm (C, D) and 60 μm (E, F).