Figure 1.
All twinfilins sequester predominantly ADP-G-actin at steady state in solutions of F-actin containing ATP. Polymerization of actin (2.5 μM) was seeded by gelsolin-actin (6 nM), with the following additions: black: none; blue: 5 μM thymosin β4; red: 5.4 μM mammalian twinfilin; green: 5.1 μM yeast twinfilin.