Figure 7.

MDMX nuclear import after DNA damage requires a cryptic NLS. (A) GFP–MDMX fusions were stably transfected into U2OS cells and photographed for localization using fluorescence from the GFP tag. (B) Full-length MDMX with the K468E mutation was transiently transfected into U2OS cells, treated for 18 h with 0.5 μM CPT, and stained using 8C6 antibody. (C) GST-importin-loaded beads were incubated with lysate of HCT116 cells transiently transfected with wild-type or mutant MDMX. Bound MDMX were detected by Western blot. (D) Diagram of MDMX indicating the location of the cryptic NLS.