Figure 4.

Immunolocalization of meiotic proteins in spread preparations of PMCs in an Atmlh3-1 mutant. Localization of the axis-associated protein ASY1 (green) at zygotene (A) and the SC protein ZYP1 (green) at pachytene (B) appears normal, indicating that chromosome pairing and synapsis occur in the absence of AtMLH3. Dual localization of ASY1 (green) with the recombination protein RAD51 (red) (C) and ZYP1 (green) with AtMSH4 (red) (D) suggests that recombination progresses to the dHj stage. However, normal localization of AtMLH1 fails to occur. Dual localization with ASY1 (green) at leptotene reveals aberrant nucleolar localization of AtMLH1 (red) (E). At pachytene, ZYP1 (green) is present but there is a complete absence of AtMLH1 foci (F). Bar=10 μm.