Figure 8. Coalescence within monocytes of two different-colored microspheres coming from two separate and independent loops of the manipulated small intestine. A two-loop small intestinal model was created to investigate the luminal transference pathway of the microspheres. The selective injection of yellow and red microspheres into individual intestinal loops and their combined appearance within extravasated monocytes within a single muscularis whole mount indicated that the microspheres do not transmurally pass through the gut wall to the muscularis, but that a more indirect extraintestinal route is taken.