Figure 2. Tissue sections stained with hematoxylin and eosin. Representative histologic appearance of livers subjected to either 75 or 120 minutes of ischemia and 3 hours of reperfusion. Normal parenchyma was observed after ischemic insults of 75 and 120 minutes using intermittent clamping and after 75 minutes with preconditioning. Severe parenchymal alterations were observed in the control groups (75 and 120 minutes of continuous ischemia only) and with ischemic preconditioning in livers subjected to 120 minutes of ischemia.