Figure 1. The generation of Sur/SSTR5 double knockout (KO) mice. SSTR5 KO and Sur1 KO mice were crossbred to yield double heterozygous animals. These animals were inbred and screened with polymerase chain reaction to detect mice that were homozygous null for both SSTR5 and for Sur1. These mice were then put into a separate colony to breed and age.