Figure 2. Polymerase chain reaction analysis of SSTR5 knockout (KO) mice and Sur1 KO mice. Tail DNA was combined with appropriate primers and underwent polymerase chain reaction amplification. Fifteen microliters was run on a 1% agarose gel with appropriate molecular markers. (A) SSTR5 primers result in a 280-bp band for the wild type, a 525-bp band for the KO, and both for a heterozygote. (B) Sur1 primers result in a 524-bp band for the wild type, a 350-bp band for the KO, and both for a heterozygote.