Figure 2. Production of monocyte chemoattractant protein 1 (MCP-1) in cultures of splenic macrophages from mice early after thermal injury. (A) Splenic macrophages (2 × 106 cells/mL) from normal mice or thermally injured mice 12 hours after thermal injury (1/2D-Mφ) were cultured for 24 hours in fresh complete medium without any stimulation. (B) Splenic macrophages, prepared from mice 6, 12, 18, and 24 hours after thermal injury, were cultured for 24 hours without any stimulation. Culture fluids obtained from both experiments were assayed for MCP-1 by enzyme-linked immunosorbent assay. Each result is displayed as mean ± standard deviation (n = 5).