FIGURE 2. Decreased TH1 and TH2 cytokine expression in CD4+ T cells but decreased TH1 cytokine expression only in CD8+ T cells from trauma patients compared with control subjects on day 7 after injury. (A) Decreased SEB-induced IFNγ, IL-2, IL-4, and IL-10 intracellular expression in CD4+ T cells from patients compared with control subjects. (B) Decreased SEB-induced IFNγ and IL-2 but not IL-4 nor IL-10 intracellular expression in CD8+ T cells from patients compared with control subjects. Data are expressed as the mean ± sem. †P < 0.05; *P < 0.01; and #P < 0.001 for patients versus control subjects. (C and D) FACS plots of SEB-induced IL-4 and IL-2 intracellular expression and IgG isotype control in the gated T cell subsets. Results are representative of a trauma patient compared with a healthy control subject. The value shown is the upper right quadrant indicates the percentage of cytokine-positive cells.