Figure 2. Purification of BmrA as analysed by PAGE.

(A) SDS/PAGE. Lane 1, molecular mass markers; protein standards in kDa are indicated on the left side. Lane 2, protein eluted from the Ni²⁺-High Trap chelating column during the 240 mM imidazole step. Lane 3, protein eluted by SEC/FPLC on a Superdex 200 10/300 GL column at 8.9 ml. Lane 4, protein eluted by SEC/FPLC on a Superdex 200 10/300 GL column at 11.5 ml. (B) Non-denaturing PAGE of fractions eluted by SEC/FPLC on a Superdex 200 10/300 GL column. Lane 5, BmrA eluted at 8.9 ml. Lane 6, BmrA eluted at 11.5 ml.