Figure 4. SEC of AlgE1- and AlgE6-epimerized polyMG (F_G=0.55 for both enzymes) specific degraded by M-lyase from H. tuberculata.

The oligo-uronic acids were chromatographed on three columns of Superdex 30 at a flow rate of 0.8 ml/min with 0.1 M ammonium acetate at room temperature. The eluent was monitored on-line with a refractive index detector. The DP and composition (sequence) of marked peaks [I−IV (E1) and (E6)] are given in Table 1. RI, refractive index.