Table 3. Epimerization characteristics of AlgE1-1 and AlgE1-2.

Enzyme activity on different substrates of the two separate parts of AlgE1, AlgE1-1 (A₁R₁R₂R₃) and AlgE1-2 (A₂R₄) expressed separately in E. coli [10]. Samples were analysed by ¹H-NMR (300 MHz) spectroscopy, in ²H₂O at 90 °C.

		AlgE1-1 (A1R1R2R3)			AlgE1-2 (A2R4)
Substrate	Reaction time (h)	FG	FGG	FGM	FG	FGG	FGM
Mannuronan	0	0.00	0.00	0.00	0.00	0.00	0.00
	3	0.67	0.54	0.13	0.37	0.00	0.37
	23	0.72	0.61	0.11	0.42	0.00	0.42
PolyMG	0	0.46	0.00	0.46	0.46	0.00	0.46
	3	0.46	0.00	0.46	0.46	0.00	0.46
	23	0.46	0.00	0.46	0.46	0.00	0.46
L. hyperborea leaf	0	0.49	0.33	0.16	0.49	0.33	0.16
	3	0.65	0.47	0.18	0.59	0.36	0.23
	23	0.69	0.51	0.18	0.59	0.35	0.24