Table 1. Substrate specificity of recombinant E. coli KpsF.

Substrates were assayed at 10 mM with 1 mM EDTA in 50 mM Tris/HCl (pH 7.8) for 10 min with 90 nM KpsF at 37 °C.

Substrate	Absorbance ratio*
A5P	2.5±0.1
D-Ribose 5-phosphate	<1
D-Arabinose	0.96±0.06
D-Glucose 6-phosphate	1.01±0.05
D-Mannose 6-phosphate	0.94±0.06
α-D-Glucose 1-phosphate	1.00±0.09
D-Glucosamine 6-phosphate	1.01±0.01

* Ratio of absorbance at 540 nm of the sample to that of the control.