Table 3. MT protein levels in rotenone-, metal- and t-BHP-treated HeLa cells.

Total metallothionein (MT1+MT2) levels are expressed relative to total protein content in cell homogenates (10 mM Tris/HCl, pH 8.0). Mean values are shown with ranges in parentheses (n=2, except metal controls where n=1).

Treatment	MT (ng/mg)
Untreated	34.7 (32.3–37.1)
Rotenone
100 nM	47.0 (44.1–49.8)
500 nM	52.1 (51.3–52.8)
1000 nM	53.8 (53.1–54.4)
2500 nM	71.7 (71.1–72.4)
Metal controls
CdCl2 (12.5 μM)	875
ZnCl2 (250 μM)	481
t-BHP
0.5 mM	56.2 (47.7–64.5)
0.8 mM	61.9 (57.6–66.2)
1.0 mM	91.5 (86.1–96.8)