Cells were cultured in 25 mM/25 mM or in 0 mM/5 mM glucose for 2 weeks post-confluence. (A) Glycogen, stained black, is indicated by arrows. MV, microvilli; N, nucleus; Gly, glycogen. Scale bar, 500 nm. (B) GLUT2, glucose-6-phosphatase (G6Pase) and L-pyruvate kinase (L-PK) mRNA levels in cells cultured as indicated. Results shown are the means±S.E.M. for three experiments performed in triplicate. The asterisk (*) indicates significant differences (P<0.05) from the values for Caco-2 cells cultured in the 25 mM/25 mM glucose condition for 2 days post-confluence.