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. 2006 Mar 28;395(Pt 2):393–403. doi: 10.1042/BJ20051359

Figure 6. TG content in the secretory compartment of Caco-2 cells cultured in 25 mM/25 mM or 0 mM/5 mM glucose for 2 weeks after confluence.

Figure 6

(A) Electron micrographs showing the presence of lipid droplets in the secretory compartment. Cells were incubated for 2 h with complex lipid micelles. Arrows show lipid droplets within the ER and the Golgi apparatus (G). MV, microvilli; N, nucleus. Scale bar, 500 nm. (B) TLC of lipids present in whole microsomes, and in the membranes and the lumen of microsomes. Cells were incubated for 3 h with lipid micelles containing [1-14C]OA and BA. An autoradiography of a representative TLC is shown. (C) Quantification of the labelled TG (upper panel) and phospholipid (PL; lower panel) present in the microsomal fractions after separation by TLC. The fractions were isolated from cells cultured in 25 mM/25 mM (white bars) or 0 mM/5 mM (black bars) glucose. Results are expressed as c.p.m. per μg of protein in each fraction. Results shown are the means±S.E.M. for three independent experiments. The asterisk (*) indicates a significant difference (P<0.05) from the value for Caco-2 cells cultured in 25 mM/25 mM glucose condition. Note: triglycerides=TGs.