Table 1. Quantification of TGs, ApoB100 and ApoB48 in lipoprotein fractions.

Caco-2 cells were grown in medium containing 25 mM glucose in both compartments until confluence and maintained for 2 weeks more in the same condition (25 mM/25 mM) or switched to media containing 0 and 5 mM glucose in the apical and basal compartment respectively (0 mM/5 mM). On the last day of culture, cells were incubated for 24 h in the presence of complex micelles containing either [1-¹⁴C]OA (1 μCi/ml), for TG analysis, or [³⁵S]methionine/cysteine (100 μCi/ml), for ApoB analysis. Basolateral media were fractionated by sequential ultracentrifugation and lipoprotein fractions were recovered. For TG analysis, lipids were extracted from lipoprotein fractions, separated by TLC and radioactive spots were counted. For ApoB analysis, lipoprotein fractions were analysed by SDS/6% PAGE and placed against phosphor screens for quantification of the individual bands corresponding to ApoB100 and ApoB48. The total secretion of TG, ApoB100 and ApoB48 by cells cultured in 25 mM/25 mM glucose was arbitrarily set to 100. Results shown are the means±S.E.M. for three independent experiments and are expressed in terms of arbitrary units. d, density; VLDL, very-low-density lipoprotein. *Indicates significant differences (P<0.05) from the values for Caco-2 cells cultured in 25 mM/25 mM glucose condition.

		d<1.006
	Total	Chylomicron	VLDL	1.006<d<1.063	1.063<d<1.21
TG
25 mM/25 mM	100	2.6±1	30.7±3	61.6±4	5±1
0 mM/5 mM	206.4±19*	9.6±4	74.4±15*	114.7±6*	7.8±1
Fold increase	2.1±0.2	3.3±0.7	2.5±0.7	1.9±0.1	1.6±0.1
ApoB100
25 mM/25 mM	100	1.9±0.8	31.5±10	62±12	4.6±1.6
0 mM/5 mM	144±17	3.1±1	53.3±15	83±5	4.7±1.7
Fold increase	1.4±0.2	1.8±0.3	1.8±0.2	1.5±0.4	1.1±0.2
ApoB48
25 mM/25 mM	100	0.8±0.3	13±4.2	51.3±11	34.9±6.8
0 mM/5 mM	129±12	2.2±1.3	20.8±5.3	64.2±11	41.6±5.9
Fold increase	1.3±0.1	2.5±0.6	1.7±0.2	1.3±0.2	1.3±0.4