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. 2006 Mar 28;395(Pt 2):277–284. doi: 10.1042/BJ20051964

Figure 1. Colony formation of CHO cells incubated with m-tyrosine.

Figure 1

Exponentially growing CHO cells were plated on to tissue culture dishes and incubated for 4 h to allow them to attach to the surface. The cells were then exposed to the indicated final concentrations of amino acid in medium A [a 1:1 (v/v) mixture of Ham's F-12 medium and Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum, penicillin, streptomycin, 2 mM L-glutamine and 1 mM sodium pyruvate] and incubated for 7–10 days. The resulting cell colonies were stained with Methylene Blue and counted. Survival curves were constructed by plotting the surviving fractions of cells (number of colonies counted/number of cells seeded)×(colony efficiency of the control cells). Results are means±S.D. for three independent experiments.