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. 2006 Mar 28;395(Pt 2):433–441. doi: 10.1042/BJ20051597

Figure 6. Effects of recombinant SmSds on PP1 activity.

Figure 6

(A) Different amounts of recombinant PP1 (7000 units/mg) ranging from 0.5 to 5 units per assay were pre-incubated for 1 h at room temperature with different concentrations of SmSds before testing for phosphatase activity in a final volume of 200 μl. −, Activity in medium alone (100%); ♦, relative activity of PP1 in the presence of GST protein control (no inhibition was observed whatever the concentration used). Only the effect of GST on 0.5 unit of PP1 is represented; no significant effects were observed on 2 and 5 units of PP1 (results not shown). ▲, ● and ■ represent the relative activity of 5, 2 and 0.5 units respectively of PP1 in the presence of different concentrations of recombinant SmSds. (B) Effects of OA on PP1 activity. ▲, ● and ■ represent the relative activity of 5, 2 and 0.5 units respectively of PP1 in the presence of different concentrations of OA. Results are means±S.D. for three representative experiments performed in triplicate.