Figure 8. SmSds expression in Xenopus oocytes and its interaction with endogenous PP1.

(A) Expression of SmSds protein by Xenopus oocytes microinjected with SmSds cRNA. Immunoblot analysis was performed on oocytes injected with vehicle (lane 1) or SmSds cRNA (lane 2). The arrow indicates SmSds. Molecular-mass sizes are given in kDa. (B) Interaction of SmSds with Xenopus PP1. Immunoblot analysis of eluates from oocytes extracts after binding to microcystin–agarose beads, revealed using either anti-PP1 antibodies or anti-SmSds antibodies. The two left-hand lanes correspond to SmSds cRNA-injected oocyte eluates, and the two right-hand lanes correspond to eluates of vehicle-injected oocytes.