Abstract
The antigenic properties of some synthetic polymers containing hydroxyproline and acetyl-hydroxyproline have been tested in guinea-pigs and rabbits by active cutaneous anaphylaxis, delayed skin hypersensitivity reactions, PCA, tanned cell agglutination and fluorescent antibody microscopy.
The antigenic relationships between these polymers, collagen and acetylated collagen have been investigated. The results obtained suggest that acetyl-hydroxyproline is a common antigenic determinant in both acetylated copolymers and acetylated collagen.
Poly-hydroxyproline and poly-acetyl-hydroxyproline were found not to be antigenic in rabbits or guinea-pigs. Rabbit antiserum against acetylated collagen has been used to stain acetylated tissue sections by immunofluorescence. Absorption studies indicate that acetyl-hydroxyproline groups are important antigenic determinants as shown by the considerable decrease in specific fluorescence when the rabbit anti-acetylated collagen is absorbed with a synthetic polymer containing acetyl-hydroxyproline.
The overall results are discussed in terms of the structural similarity existing between collagen and some of the polymers used in this work.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRICK F. H., RICH A. Structure of polyglycine II. Nature. 1955 Oct 22;176(4486):780–781. doi: 10.1038/176780a0. [DOI] [PubMed] [Google Scholar]
- GREEN R. W., ANG K. P., LAM L. C. Acetylation of collagen. Biochem J. 1953 May;54(2):181–187. doi: 10.1042/bj0540181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JASIN H. E., FINK C. W., WISE W., ZIFF M. Relationship between urinary hydroxyproline and growth. J Clin Invest. 1962 Oct;41:1928–1935. doi: 10.1172/JCI104650. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JASIN H. E., GLYNN L. E. THE ANTIGENICITY OF SOME SYNTHETIC POLY-IMINOACIDS. I. ANTIGENIC PROPERTIES OF POLY-L-PROLINE. Immunology. 1965 Jan;8:95–105. [PMC free article] [PubMed] [Google Scholar]
- LOEWI G., GLYNN L. E., DORLING J. Studies on the nature of collagen degeneration. J Pathol Bacteriol. 1960 Jul;80:1–8. [PubMed] [Google Scholar]
- PROCKOP D. J., UDENFRIEND S. A specific method for the analysis of hydroxyproline in tissues and urine. Anal Biochem. 1960 Nov;1:228–239. doi: 10.1016/0003-2697(60)90050-6. [DOI] [PubMed] [Google Scholar]
- RAM J. S., MAURER P. H. Immunochemical studies on some acetylated proteins; acetylated rabbit serum albumin, an antigen in rabbits. Arch Biochem Biophys. 1957 Nov;72(1):119–126. doi: 10.1016/0003-9861(57)90179-0. [DOI] [PubMed] [Google Scholar]
- RAM J. S., MAURER P. H. Modified bovine serum albumin. IV. Characterization and immunochemical studies of the O-acetylated derivative. Arch Biochem Biophys. 1958 Mar;74(1):119–130. doi: 10.1016/0003-9861(58)90206-6. [DOI] [PubMed] [Google Scholar]
- SCHMITT F. O., LEVINE L., DRAKE M. P., RUBIN A. L., PFAHL D., DAVISON P. F. THE ANTIGENICITY OF TROPOCOLLAGEN. Proc Natl Acad Sci U S A. 1964 Mar;51:493–497. doi: 10.1073/pnas.51.3.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELA M., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 3. The role of rigidity in the antigenicity of polypeptidyl gelatins. Biochem J. 1960 Nov;77:394–399. doi: 10.1042/bj0770394. [DOI] [PMC free article] [PubMed] [Google Scholar]