Abstract
The Coons' technique has been modified for the detection of antibodies on erythrocytes agglutinated by fluorescein-conjugated antisera. The results of experiments with several haemagglutinating systems suggested that specific fluorescence was found only on erythrocytes which had been agglutinated by species-specific antibodies. The significance of these findings is discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOURSNELL J. C., COOMBS R. R., RIZK V. Studies with marked antisera; quantitative studies with antisera marked with iodine 131isotope and their corresponding red-cell antigens. Biochem J. 1953 Dec;55(5):745–758. doi: 10.1042/bj0550745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]