Figure 5.

Localization and recycling of epitope-tagged furin. Parallel plates of BSC-40 (A and B), M2 (C and D), and A7 cells (E and F) grown on glass coverslips were infected with VV:hfur/f (moi = 10). At 4 h after infection, mAb M1 (6 μg/ml final concentration) was added to the culture media for an additional hour. The cells were then fixed and permeabilized, and the samples were incubated with mAb M2 to detect the steady state distribution of furin staining. The samples were then processed for immunofluorescence microscopy. The mAb M1 was detected using a goat anti–mouse IgG_2b-FITC secondary antibody (A, C, and E) and mAb M2 was visualized using a goat anti–mouse IgG₁-TXR antibody (B, D, and F). The exposure time in C was adjusted to equal the exposure time in E to compare directly the intensity of fluorescence staining between samples.