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Colocalization of fur/f and γ-adaptin. Replicate plates of M2 (A and C) and A7 (B and D) cells were infected with the vaccinia recombinant expressing fur/f (moi = 10). At 4 h after infection, the cells were fixed with paraformaldehyde, permeabilized, and incubated with mAb M2 to detect fur/f (A and B) and 100/3 to detect γ-adaptin (C and D). The mAb M2 was visualized with anti–mouse IgG1-TXR and mAb 100/3 was visualized with IgG2b-FITC.
