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. Author manuscript; available in PMC: 2007 Jan 15.
Published in final edited form as: J Immunol. 2006 Jan 15;176(2):811–818. doi: 10.4049/jimmunol.176.2.811

FIGURE 6.

FIGURE 6

Egr-1 is required for CD154 message induction. T cell transgenic primary murine CD4 T cells from Egr-1 WT (+/+) or knockout (−/−) mice were rested (−) or stimulated (+) in vitro for 5 h with the appropriate peptide/MHC combination (see Materials and Methods). mRNA was isolated and analyzed by Northern blot with a probe specific to murine CD154 cDNA. The blot was then stripped and probed for the GAPDH housekeeping gene as a loading control. Results are representative of one of two similar experiments.