Skip to main content
. Author manuscript; available in PMC: 2006 Apr 3.
Published in final edited form as: Mol Ther. 2006 Feb 7;13(4):756–765. doi: 10.1016/j.ymthe.2005.12.008

Figure 2.

Figure 2

Biodistribution of Ad mediated transgene expression and vector genomes in C57 and C57-CD46 mice after intramuscular injection of Ad5.GFP and Ad5/35.GFP. A) GFP expression in organ sections 72 hours after intramuscular injection of 5×109 pfu of Ad5.GFP and Ad5/35.GFP. Tissue sections were analyzed for GFP expression by immunohistochemistry with anti-GFP antibodies. B) Quantitative comparison of viral genomes present in major organs at 72 hours after intramuscular Ad injection. The genome concentration was expressed as the number of viral genomes per 107 cells (assuming that the mass of a diploid human genome is 6pg). qPCR results for vector genomes were equalized based on qPCR data for an endogenous (two copies/genome) mouse GAPDH gene. The average of 3 independent tissue samples is shown. Standard deviation was less than 10%C) Detection of Ad5/35 particles around the injection site. Mice were injected IM with 5×109pfu Ad5/35.GFP in 40μl of PBS. One hour after injection, mice were sacrificed. Sections (8μm) of muscle tissue around the injection site were analyzed by immunofluorescence. Viral particles were detected using anti-Ad5 hexon-FITC antibodies (green). Muscle cells were stained with anti-laminin antibodies (red). Cell nuclei are blue.