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A. Molecular mass determination of intact flagellin from the complemented strain.
B. Second generation product ion spectra of oxonium ion m/z 486 obtained from in source dissociation of a multiply charged flagellin ion. The presence of m/z 316 product ion confirms the restoration of both groups on this flagellin (see Schirm et al., 2005). Conditions used for in source fragmentation are as described in figure 3.
