Table 1.
Genes in the flagellin glycosylation locus of C. jejuni 81–176.
| NCTC homolog1 | Gene/annotation2 | Homology to 111683 | length bp | Position of insertion4 | Phenotype of mutant | Reference |
|---|---|---|---|---|---|---|
| Cj1292 | dcd; dCTP deaminase | 98/99 (186) | 561 | N/A | ND | N/A |
| Cj1293 | pseB | 94/98 (334) | 1005 | 395 NP | mot− | Goon et al., 2003 |
| Cj1294 | pseC | 88/91 (376) | 1131 | 654 NP | mot− | this work |
| Cj1295 | amidopeptidase | 95/97 (233)5 | 708 | 335 P | mot+; wt IEF | this work |
| Cj1300c | unknown | 50/68 (295) | 897 | 287 NP | mot+; wt IEF | this work |
| Cj1298 | unknown | 46/63 (234) | 720 | 259 NP | mot+; wt IEF | this work |
| Cj1299 | accP | 72/85 (75) | 228 | ND | NA | NA |
| None (CJB1301) | NA | none | 1359 | 807 NP/663 P | mot+; wt IEF | this work |
| Cj1302 | unknown | 95/95 (520) | 1563 | 122 P | mot+; wt IEF | this work |
| Cj1303 | fabH2 | 99/99 (353) | 1062 | 302 NP | mot+; wt IEF | this work |
| Cj1304 | accP | 95/97 (73) | 221 | 81 NP | mot+; wt IEF | this work |
| Cj1305c | unknown | 81/86 (405) | 1215 | 271 NP | mot+; wt IEF | this work |
| Cj1311 | pseF | 97/98 (232) | 699 | 304 P | mot− | this work |
| Cj1312 | pseG | 92/92 (198) | 597 | 304 P | mot− | this work |
| Cj1313 | pseH | 88/95 (127) | 384 | 246 P | mot− | this work |
| Cj1314c | probable cyclase | 93/93 (248) | 747 | 134 NP | mot+; wt IEF | Thibault et al., 2001 |
| Cj1315c | amidotransferase | 98/99 (201) | 606 | 205 NP | mot+; wt IEF | Thibault et al., 2001 |
| Cj1316c | pseA | 99/99 (378) | 1137 | NA | mot+; PseAm− | Thibault et al., 2001 |
| Cj1317 | pseI | 92/93 (343) | 1032 | NA | mot− | Thibault et al., 2001 |
| Cj1334 | unknown (maf3) | 71/84 (544) | 1638 | 817 P | mot+; wt IEF | this work |
| Cj1333 | pseD | 64/79 (653) | 1965 | 1407 NP | mot+/PseAm−; 486−; 487+ | this work |
| Cj1337 | pseE | 61/76 (653) | 1899 | 998 NP | mot− | this work |
| Cj1338c | flaB | 75/81 (576) | 1731 | NA | N/A | this work |
| Cj1339c | flaA | 75/81 (576) | 1731 | NA | N/A | NA |
| Cj1341c | unknown (maf6) | 58/72 (605) | 1827 | 1121 NP | mot+; wt IEF | this work |
| Cj1342c | unknown (maf7) | 71/80 (404) | 1251 | 1062 NP | mot+; wt IEF | this work |
The NCTC 11168 that showed the highest homology to 81–176 is listed; as stated in the text, some genes in 81–176 are in a different order than those in NCTC 11168;
Annotations are the original annotations from Parkhill et al. (2000), Karlyshev et al., (2002) or from this work based on loss of PseAm or lack of motility suggesting a role in PseAc synthesis or attachment; annotations in parentheses indicate motility associated genes in NCTC 11168 but not 81–176;
homology to NCTC11168 was done by blastp analysis at campyDB (http://campy.bham.ac.uk/) and is given at the % identity/% similarity (number of amino acids);
position of the transposon insertion was determined by sequence analysis in E. coli and is given from the ATG start in bp;
The 81–176 allele of Cj1295 was truncated due to a possible slip strand mismatch at a homopolymeric tract of As; if the slip strand was corrected, the predicted protein showed 94% identity/96% similarity to the NCTC 11168 gene over 418 amino acids.