Table 1.

PCR Primers and Conditions Used for RAB3GAP2 Sequencing^[Note]

	Primer(5′→3′)
Exon(s)	Forward	Reverse	Product Size	MgCl2(mmol/liter)
1	GCGAGTAGGAGACTCGAACC	GGCTAGAGCTATTGGGGCTC	290	1
2	AAAATGTCAGCTTGACAGGG	TCAGCATTATACAGGGGTTGG	260	1
3	CACCTAATCCATTTTCTCTTCTATCC	CTCTCACCCCTTGAGACAGG	275	1.5
4	TCACCTACAAGTCAAATAATTTCATAC	AATCATATTCTAAGGGGAAAGAGC	246	1
5	CACAATGCAGGTCCAGTCAG	GCATCAAATAAAACTGGTTCACAG	288	1
6–7	GCTAACTAACTGTGCCACAAGAC	TCCCACTAGCAAGCCAAAAG	706	1
8	GACATACTTAATTCCATAAGCAGGTG	GGGTGTTGGGTGGTTATGTTAC	286	1
9	CTTGATCCTGGAAGGTGGAG	ACGTGCATTGTCTTGGTGTG	464	1.5
10	AAGGCTAAGCAAGTTACCATATCC	TTTAGCATCTTAGAATGGAACTGAAG	321	1
11–12	TCAGCAAATCAACCAAGCAC	CTTTTCAAAGCATTTTAAAGTAAGC	484	1
13	AACATTGCTCAACTATTGGGG	GCCTATTTCATTTATGCATTTCG	293	1
14	TACATGCCAGGCACTCACAG	TAGCTTTTCTTACCCCACCC	482	1
15	CAAACCTAGGAACTAGGAAAAGTTAGC	TGATAATAATGTTGGGTTTCCTAGC	305	1
16–17	TTCAGCCTCTCATGTTTGGG	TGTTGTTTCCTTTGTCTTTAGACTG	684	1
18	CACATTAAAGTAACATGATGGTATCAC	TGGGGTAGTGAAAGTTTGGG	289	1
19	AGCACAGAGAGCTAGCAGGG	TGAAAGCAAAGCCTGTTTATG	350	1
20	TGCTATATGTTCCTACCATGTTTTC	TTTCAGTTCAGATAATGTGGGTG	350	1
21	CTGACACTTCTGGGTGACATTC	TTGTGATTCAATAAGATTGTTAGTCC	233	1
22	TTTTGTGACATTTCAAACACG	GCTGTCTAGGAAAGTGTAAGATTTTG	238	1
23	AAGGAAAGCATCCCCTTAGC	TTGATTCAGAACAAAGATGACTAAAAG	434	1
24	CTGAGCGTGGAACTGACC	ACCCTCATGTTTCATCCAGC	578	1
25–26	GCTATTTAATTAGACGGTCCCC	AAACACTGCCCTGTGTGTTG	522	1
27	TCTTTAAATTCACATCCTGTTAAATC	TCTCCATTGATAATGGTAAAACC	219	1
28	TGAGGATTTAGAGTCCTTCTTTATG	TTCATGCTTAGTGGCAATTTAGAC	493	1.5
29	AATGATAAAAGGCAAAGTTCACC	ACTTATAGCGCCATTTACGG	247	1
30	TGCAATGGTTTGCAGAATATG	TTTGGCTGACAGTAACTATAGCAG	206	1
31	AAATATGAAAATCCATGTGTCTGC	ATGAATTTGCACTTTGCTGC	387	1
32	TGTCCTTGCGATAGAAAGTAGTC	GGGGATCACTGGGTTTCATC	391	1
33	GCAAAAGGCTTCATTGGAAAC	TGACAGAAAAGCTAACAATACTTGG	402	1
34	CCAGGCACTCAAGAAAACATC	CTTCACTGGCAACCCCTTTC	299	1.5
35_1	CAGCTGTGAGGTAGGTTTGC	GCTGCCACCTGGGAGTTC	470	1
35_2	TGAACCCATTCTTCCTCCTC	GACCTGGAATTTTATGGGAAAAG	513	1

Note.— Annealing temperature was 60°C for all sequencing.