Fig. 1.
Microtubule-associated serine/threonine kinase with a molecular mass of 205 kDa (MAST205) and its derivatives used in the study. MAST, 5B, and BP are in pcDNA1-neo, whereas MΔ5, KMΔ5, and PC are cloned in pcDNA3.1-HisB. KD is cloned in pET30. The numeric values indicate the amino acid numbers. W78 and W98 are the partial human MAST205 genes identified by yeast two-hybrid screen in pJG4–5. The location of the K482M mutation in KMΔ5 is marked. See text for definitions.