Figure 4.

Nt CMPG1 Is Required for Efficient HR Mediated by Cf-9 in N. benthamiana.

(A) VIGS in N. benthamiana using TRV. Seedlings were inoculated with TRV:Nt CMPG1, TRV:Nb SGT1, or empty vector as a control (TRV:00). Three weeks after inoculation, leaves were infiltrated with agrobacteria delivering Cf-9 and Avr9. Pictures were taken 4 d after agrobacteria infiltration.

(B) RT-PCR from silenced plants. N. benthamiana leaves silenced with the indicated TRV construct were infiltrated with water to induce Nb CMPG1 expression. Samples were collected 30 min after infiltration, and RNA was isolated and used for RT-PCR. Nb ACIK1 was used as a control for induction by flooding (bottom panel).