Figure 5.

Overexpression of Nt CMPG1 Induces a Stronger HR Response to Avr9 in Cf9 Tobacco Plants.

(A) HR in N. tabacum. Cf9 tobacco plants were transformed with 35S:Nt CMPG1:TAP and analyzed for Avr9-dependent HR production. Tobacco leaves from three independent transgenic lines and a control were infiltrated with a 1/16 dilution of IF(+Avr9). Pictures were taken 3 d after infiltration.

(B) Nt CMPG1 protein levels were analyzed by protein gel blots. Leaf discs were harvested from different Cf9 tobacco lines expressing 35S:Nt CMPG1:TAP or control leaves. Total proteins were separated in SDS gels and analyzed by inmunoblotting using PAP antibody for Nt CMPG1:TAP detection. Bottom panel shows Ponceau S Red staining of ribulose-1,5-biphosphate carboxylase/oxygenase for confirmation of equal loading.

(C) Growth inhibition assay. Cf9 tobacco seedlings expressing Nt CMPG1:TAP (line J) and control were transferred to MS medium containing the indicated amount of IF(+Avr9) (+) or IF(−Avr9) (−) (μL per mL of MS medium). Pictures were taken 10 d after transfer to MS medium.

(D) Growth inhibition assay. Cf9 tobacco seedlings were transferred to MS medium containing IF(−Avr9) or IF(+Avr9) as described in (C). Ten days after the transfer, the same number of seedlings was weighted for each treatment. The percentage was calculated based on the weight of seedling growth in IF(−Avr9) containing MS medium.