Table 2.
Sterol Compositions in 35S:CYP710A1-, 35S:CYP710A2-, and 35S:CYP710A11-Transgenic Arabidopsis Lines
| Sample | 24-Methyl-Δ22-Sterolsa | 24-Methyl-Sterolsa | Stigmasterol | β-Sitosterol |
|---|---|---|---|---|
| Wild type | 0.85 ± 0.062 | 23 ± 2.4 | 3.4 ± 0.52 | 250 ± 20 |
| At11 | 1.9 ± 0.62 | 19 ± 1.5 | 110 ± 9.9 | 81 ± 3.7 |
| At12 | 0.22 ± 0.052 | 19 ± 2.1 | 21 ± 4.5 | 160 ± 6.9 |
| At13 | 0.50 ± 0.010 | 21 ± 3.2 | 86 ± 16 | 140 ± 9.1 |
| At21 | 15 ± 1.4 | 4.5 ± 0.33 | 81 ± 7.2 | 170 ± 3.8 |
| At22 | 16 ± 0.22 | 2.5 ± 0.20 | 83 ± 9.1 | 110 ± 13 |
| At23 | 14 ± 1.8 | 4.1 ± 0.44 | 50 ± 7.7 | 120 ± 5.8 |
| Se1 | 0.66 ± 0.16 | 17 ± 0.43 | 27 ± 5.8 | 190 ± 27 |
| Se2 | 0.63 ± 0.10 | 20 ± 2.6 | 27 ± 3.6 | 190 ± 27 |
| Se3 | 1.20 ± 0.20 | 26 ± 0.28 | 98 ± 29 | 190 ± 61 |
| Se4 | 0.96 ± 0.067 | 20 ± 3.5 | 46 ± 2.5 | 170 ± 33 |
| Se5 | 0.60 ± 0.053 | 16 ± 1.2 | 22 ± 4.9 | 140 ± 20 |
| cyp710a2 | n.d.b | 20 ± 1.1 | 3.7 ± 0.72 | 170 ± 14 |
The same plant samples were divided into two parts for the sterol analyses and the RT-PCR to monitor the transgene expression levels (Figure 5). Data are the means ± sd of triplicate determinations. Values are given in μg/g fresh weight.
a
The C24-epimers were not separately analyzed. 24-Methyl-Δ22-sterols are a mixture of brassicasterol and crinosterol, and 24-methyl-sterols consist of 24-epi-campesterol and campesterol.
b
n.d., not detected.