Figure 11.

Sucrose Density Gradient Analysis of LPA1 in Thylakoids.

Thylakoids (0.5 mg/mL) were solubilized with 1% DM and separated in a linear 0.1 to 1 M sucrose gradient. Twenty fractions were collected from the gradients, and the proteins from each fraction were separated by SDS-PAGE. After electrophoresis, the separated proteins were immunodetected with the antibody directed against LPA1. To identify the positions of the major photosynthetic complexes, blots were also probed with anti-D2, anti-CP47, anti-LHCII, and anti-PsaA/B antibodies. The positions of 669-kD (thyroglobulin), 440-kD (ferritin), 232-kD (catalase), 140-kD (lactate dehydrogenase), and 66-kD (BSA) molecular size markers are indicated.