Figure 12.

Interaction of LPA1 with the Reaction Center D1 Protein of PSII.

(A) β-Gal activity of the transformants expressing LPA1-Cub-LexA-VP16 together with the NubG-X (where X represents D1, D2, cytochrome b6, or Alb3) fusion proteins. Cells were grown on SD-His-Leu-Trp plates, transferred to Whatman filters, permeabilized, and incubated in the presence of 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal).

(B) Growth of the cells expressing LPA1-Cub-LexA-VP16 with various NubG-X constructs on SD-His-Leu-Trp plates. Cells were grown to logarithmic phase, and 5-μL portions of 1:10 series dilutions were spotted on the SD-His-Leu-Trp plates and incubated at 30°C for 2 d. As a positive control, NMY32 was transformed with the NubI-Alg5 expression plasmid, and as a negative control, NMY32 was transformed with the plasmid expressing NubG-Alg5.